Energy dependence of site-specific radiation damage in protein crystals
نویسندگان
چکیده
منابع مشابه
Dose dependence of radiation damage for protein crystals studied at various X-ray energies.
Radiation damage to protein crystals is the most serious problem in obtaining accurate structures from protein crystallography. In order to examine the photon energy dependence of radiation damage, 12 to 15 data sets from each of nine tetragonal lysozyme crystals were collected at nine different X-ray energies (6.5, 7.1, 8.3, 9.9, 12.4, 16.5, 20.0, 24.8 and 33.0 keV) using beamline BL41XU at SP...
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Crystal Calorimetry in future high-energy physics experiments faces a new challenge to maintain its precision in a hostile radiation environment. This paper discusses the effects of radiation damage in scintillating crystals, and concludes that the predominant radiation damage effect in crystal scintillators is the radiation-induced absorption, or color center formation, not the loss of scintil...
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Fringes and speckles occur within diffraction spots when a crystal is illuminated with coherent radiation during X-ray diffraction. The additional information in these features provides insight into the imperfections in the crystal at the sub-micrometre scale. In addition, these features can provide more accurate intensity measurements (e.g. by model-based profile fitting), detwinning (by disti...
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Understanding protein stability is a significant challenge requiring characterization of interactions within both folded and unfolded states. Of these, electrostatic interactions influence ionization equilibria of acidic and basic groups and diversify their pK(a) values. The pH dependence of the thermodynamic stability (Delta G(FU)) of a protein arises as a consequence of differential pK(a) val...
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ژورنال
عنوان ژورنال: Journal of Synchrotron Radiation
سال: 2011
ISSN: 0909-0495
DOI: 10.1107/s0909049511005504